UCR

Institute for Integrative Genome Biology



Applied Biosystems 3730xl DNA Sequencer


Genomics Instruments

GENOMICS

Applied Biosystems 3730xl DNA Sequencer
(operated by Core Staff)

3730xl
Applications
  • This instrument is used by core personnel to sequence DNA templates
  • Sequencing of cloned inserts or PCR products is done by DNA polymerase-mediated linear amplification of templates in the presence of mixtures of dNTPs and fluorescently-labeled dideoxynucleotide triphosphates (Sanger method), followed by fractionation of the extension products by capillary electrophoresis using the 3730xl.
Specifications
  • This is a capillary sequencer capable of processing 96 template samples simultaneously; up to ~4000 samples/week.
  • Submit templates in either individual reaction tube format or cost-effective plate format.
  • Standard sequencing PCR reaction conditions include 92C denaturation, annealing at 50C and extension at 60C for a total of 30 cycles.
  • Please consult with Genomics Core staff for troublesome templates requiring different conditions.
Technical Tips

More Information

General Campus Information

University of California, Riverside
900 University Ave.
Riverside, CA 92521
Tel: (951) 827-1012

Career OpportunitiesUCR Libraries
Campus StatusDirections to UCR

Genomics Information

Institute of Integrative Genome Biology
2150 Batchelor Hall

Tel: (951) 827-7177
E-mail: Aurelia Espinoza, Managing Director

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