UCR

Institute for Integrative Genome Biology



Agilent 2100 Bioanalyzer


Genomics Instruments

DNA AND PROTEIN ANALYSIS AND QUANTIFICATION

Agilent 2100 Bioanalyzer
(operated by Core Staff)

Agilent 2100 Bioanalyzer
Applications

The Agilent 2100 Bioanalyzer is used primarily to assess the quality of DNA and RNA templates for Illumina sample prep and GeneChip analysis but can be used to examine any nucleic acid or protein for mass, purity or degradation.

  • DNA size and quantity
    High-resolution separation and quantification
  • RNA quality check with RIN for mammalian samples
    The industry standard for RNA analysis offering total RNA, mRNA, and small RNA data including RIN algorithm (RNA Integrity Number). There are RIN values possible for plant samples as well.
Specifications
  • The instrument uses microfluidics contained within a chip for electrophoresis of small sample quantities.
  • Fluorescence signals are converted to gel-like images with either bands or peaks as output.
  • A variety of commercial analysis chips are available for the instrument.
Available Kits

Below are available kits. The charge is per chip, not sample number. One microliter of sample is loaded.

Agilent RNA 6000 Nano Kit (PN 5067-1511)
12 samples/chip
Quantitative 25-500 ng/ul
Qualitative 5-500 ng/ul
Buffer compatibility: 100 mM Tris or 125 mM NaCl or 15 mM MgCl2

Agilent RNA 6000 Pico Kit (PN 5067-1513)
11 samples/chip
Quantitative +/- 30% accuracy
Qualitative 50-5000 pg/ul
Buffer compatibility: 50 mM Tris or 50 mM NaCl

 Agilent Small RNA Kit (PN 5067-1548)
11 samples/chip
Quantitative 25% CV
Qualitative 50-5000 pg/ul
Max. Buffer concentration: 10 mM Tris and 0.1 mM EDTA

Agilent DNA 1000 Kit (PN 5067-1504)
12 samples/chip
Quantitative 0.1-50 ng/ul
Sizing range 25-1000bp
Max. Salt Concentration: 250 mM for KCl or NaCl, 15 mM for MgCl2

Agilent DNA High Sensitivity Kit (PN 5067-4626)
11 samples/chip
Quantitative 5-500 pg/ul
Sizing range 50-7000bp
Max. Buffer concentration: 10 mM Tris and 1 mM EDTA

Technical Tips

This instrument is operated by Core staff; for individual applications, please contact Core Staff.


More Information

General Campus Information

University of California, Riverside
900 University Ave.
Riverside, CA 92521
Tel: (951) 827-1012

Career OpportunitiesUCR Libraries
Campus StatusDirections to UCR

Genomics Information

Institute of Integrative Genome Biology
2150 Batchelor Hall

Tel: (951) 827-7177
E-mail: Aurelia Espinoza, Managing Director

Footer