UCR

Institute for Integrative Genome Biology



Sage-Scientific Blue Pippin


Genomics Instruments

DNA SHEARING AND SIZE SELECTION

Sage Scientific Blue Pippin for DNA Fragment Isolation
(user-operated)

Sage Scientific Blue Pippin

Applications
  • This instrument can used by customers to precisely size select DNA fragments of desired length.
  • Computer controlled electrophoresis and sample gating permit fragments from about 90bp up to more than 50Kb to be isolated.
  • Samples are run on a cassette containing agarose gel.
  • The primary application of size selection of DNA is for use in preparing samples for Illumina sequencing. DNA fragments can either be size selected prior to library prep or libraries themselves can be size selected and purified away from primer dimers and other undesirable products.
  • Uses can also include miRNA isolation.
Specifications
  • For DNA size selection cassettes are available from 0.75% to 3%. Gels can be stained or unstained and with or without internal size standards. We typically use unstained gels with internal standard for Illumina sample prep.
  • The Blue Pippin has both standard and pulse field electrophoresis capability permitting a large range of fragment size selection.   
  • Cassettes can run up to 5 samples. Unused sample lanes can be used at a later time in some cases.
  • For protein size selection there are special 3% and 5% agarose gels run with 0.1% SDS.
  • For more information on available gel cassettes, please see the Sage Scientific website.
Technical Tips
  • Prior to usage customers may contact the Genomics Core to arrange instrument training although the supplied manuals are very useful.
  • Please note that we charge per usage which includes one cassette.
  • Note that we do not stock all cassette types. The most popular are 1.5% and 2% dye-free. If you anticipate only occasional usage of an uncommon cassette, we may ask that you purchase the cassettes.

Services/Instrument List


More Information

General Campus Information

University of California, Riverside
900 University Ave.
Riverside, CA 92521
Tel: (951) 827-1012

Career OpportunitiesUCR Libraries
Campus StatusDirections to UCR

Genomics Information

Institute of Integrative Genome Biology
2150 Batchelor Hall

Tel: (951) 827-7177
E-mail: Aurelia Espinoza, Managing Director

Footer