UCR

Institute for Integrative Genome Biology



Qubit 2.0 Fluorescence Reader


Genomics Instruments

DNA AND PROTEIN ANALYSIS AND QUANTIFICATION

Qubit 2.0 Fluorescence Reader for DNA, RNA and Protein
(user operated)

Qubit 2.0 Fluorescence Reader
Applications
  • This popular instrument is a fluorescence reader for individual tubes that is used in conjunction with reagents kits to quantify dsDNA, ssDNA, RNA and protein.
  • This reader is easy to use and much more sensitive that the Nanodrop and is the method of choice for quantifying Illumina libraries.
  • Since it measures fluorescence (not aborbance) the Qubit does not detect UV absorbing materials such as free nucleotides, DNA contamination in RNA preps, or RNA contamination in DNA preps.
  • The Qubit is very simple to use and reagents and standards are supplied to users who are charged a modest cost per sample.
Specifications
  • The instruments has built-in programs for dsDNA (high sensitivity), dsDNA (broad range), RNA, ssDNA, and Protein.
  • Sensitivities are: dsDNA BR (broad range) 2-1000ng; dsDNA HS (high sensitivity) 0.2-100ng; ssDNA 1-200ng; RNA HS (high sensitivity) 5-100ng; protein 1-500ng.
  • For more information on Qubit 2.0 reagents see the Life Technologies webpage.
Technical Tips

  • Reagents are supplied by the Genomics Core and included in the per sample price.
  • Although the Nanodrops are advertised as sensitive to 2ng/ml, they are not particularly accurate at such low concentrations. For samples below about 20ng/ul we would recommend the Qubit reader. It is more sensitive and is not affected by UV absorbing materials such as free nucleotides that can result in an overestimation for DNA or RNA.

More Information

General Campus Information

University of California, Riverside
900 University Ave.
Riverside, CA 92521
Tel: (951) 827-1012

Career OpportunitiesUCR Libraries
Campus StatusDirections to UCR

Genomics Information

Institute of Integrative Genome Biology
2150 Batchelor Hall

Tel: (951) 827-7177
E-mail: Aurelia Espinoza, Managing Director

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