UCR

Institute for Integrative Genome Biology



Liquid SDS Protein Fractionator


Proteomics Instruments

Liquid SDS Protein Fractionator

Liquid SDS Protein Fractionator
Applications
  • Simplify and reduce the dynamic range of complex protein mixtures for bottom-up discovery proteomics using LC-MS/MS.
  • Fractionate and recover proteins intact for top-down proteomic characterization.
  • Isolate and enrich user-selected molecular weight fractions for targeted protein quantification using LC-MS/MS.
  • Separate protein pull-down components for target protein purification.
Specifications
  • Intact protein molecular weight fractionation, isolation, and purification.
  • Liquid-phase recovery without band or spot cutting.
  • Broad mass range fractionation up to 500 kDa.
  • Up to eight samples processed in parallel.
  • Programmable fractionation for isolating and purifying targeted proteins
  • High protein recovery (>80%) and High reproducibility (<15% CV).
  • High loading capacity (>5X more than a 1D gel).
  • Proteins are recovered intact, for complete characterization.
Technical Tips
  • Permits effective, programmable fractionation, isolation, and purification across a broad mass range from 10-500 Kd.
  • Combined with its high load capacity and high, liquid-phase recovery , the system provides the highest sensitivity of any pre-fractionation technology.
  • May be operated in fractionation mode, in which the entire sample is partitioned into 1-29 selectable MW fractions, or in targeted isolation/purification mode, allowing for extraction of analytes in one or more pre-determined mass ranges.
  • The recovedred liquid protein samples can be further precipitated with acetone to produce highly pure protein pellets that are ready for trypsin digestion in proteomics studies.
Sample Uses
  • Total crude cellular proteins.
  • Any recombinant proteins.
  • Any affinity-based pull-down samples.

More Information

General Campus Information

University of California, Riverside
900 University Ave.
Riverside, CA 92521
Tel: (951) 827-1012

Career OpportunitiesUCR Libraries
Campus StatusDirections to UCR

Genomics Information

Institute of Integrative Genome Biology
2150 Batchelor Hall

Tel: (951) 827-7177
E-mail: Aurelia Espinoza, Managing Director

Footer