UCR

Institute for Integrative Genome Biology



Q-TOF ESI MS/MS


Proteomics Instruments

Q-TOF ESI MS/MS

Q-TOF closeup

Q-TOF Plot

Applications
  • Useful for complex samples containing a number of proteins
  • Excellent for studies of organelle proteome profiling and protein complexes profiling
  • Allows label-free quantitative comparison in functional proteomics
  • Good for site mapping of a variety of protein post-translational modifications
Specifications
  • Made by Micromass, a subsidiary of Waters Corporation
  • Configured with an nano-ESI ionization source
  • Coupled with a non-splitting nano-UPLC system; automatically integrates peptide separation, MS/MS analysis, data interpretation, and database search into a single run using single centralized software control
  • 10,000 psi UPLC to support ultra-high back pressure columns
  • Operates in either standard V-mode with 10,000 resolution or W-mode with 17,000 resolution
Technical Tips
  • Avoid potential contaminants such as karetins, detergents, and salts
  • Samples have to be free of debris and invisible particles that may clog the LC column and tubing
  • Sample buffers and preparation procedures need to be compatible with LC/MS.
Sample Uses
  • 1D-gel bands
  • Mixture of cellular proteins
  • In-solution digest of gel-free samples purified by small-molecule affinity

More Information

General Campus Information

University of California, Riverside
900 University Ave.
Riverside, CA 92521
Tel: (951) 827-1012

Career OpportunitiesUCR Libraries
Campus StatusDirections to UCR

Genomics Information

Institute of Integrative Genome Biology
2150 Batchelor Hall

Tel: (951) 827-7177
E-mail: Aurelia Espinoza, Managing Director

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