UCR

Institute for Integrative Genome Biology



Facility Regulations


Regulations

The instrumentation within all of the core facilities is delicate and very expensive.  When an instrument in operated improperly or is out of order, it affects all users. The Institute strives to keep costs as low as possible to support research efforts. Repairs and service contracts are extremely expensive, but if every user exercises proper care and attention when using equipment, these costs are minimized.

The concept of shared resources is important in other ways.  For example, it is important to be considerate to the needs of other users to reserve equipment only for periods when it is truly needed so that other users can use our resources equally. We are fortunate to have an excellent set of core facilities at the UCR campus with access to equipment and services that enable research.  These facilities belong to all of us, and your participation is essential to maintain the best services and equipment and meet our future research needs as well.

GENERAL

  1. If there is any uncertainty as to whether something is misaligned, broken, dirty, or not performing as it should please contact the appropriate Academic Coordinator or the Academic Administrator as soon as possible so that problems can be dealt with quickly.
  2. These are laboratories, so please refrain from bringing food, water or gum into instrument labs.
  3. To keep systems virus and malware-free, leave them disconnected from the network and do not expose them to any suspect files or programs.  No internet surfing; no personal email.
  4. SECURITY IS CRITICAL. The IIGB has many high value items including scientific equipment and computers. It is also a highly trafficked area, especially after hours. This makes our facilities more vulnerable to theft and sabotage. It is the responsibility of all users to be sure that exit doors to the building and the appropriate Core facilities are closed and locked after use. Replacement after theft or malicious intent can be extremely costly, impacting all campus users.

TRAINING

  1. All users must be trained by the Academic Coordinator or other Core staff directly, not by other users, prior to using equipment at a Core Facility. If you are already experienced at using an instrument, you still need to confirm with the Academic Coordinator that you can handle the instrument correctly; then learn local operating procedures in a supervised session before using an instrument on your own.
  2. To request training, please contact the appropriate Academic Coordinator and provide a valid FAU (full accounting unit) number for subsequent billing of equipment usage. This number can be obtained from the principal investigator in your lab. An academic coordinator will contact you regarding your training/equipment usage request and training requirements.
  3. If you have already completed an online personal profile form, please view the IIGB Calendar for information regarding upcoming workshops and procedures for enrollment.

SAFETY

Chemical Safety
  1. For MSDS chemical safety information, a computer in the Microscopy Core (Room 1005) is networked. Material Safety Data Sheets (MSDS) are available at www.actiocms.com/chemquik by searching the chemical name.  The room is also equipped with a printer.
  2. In addition, several computers in the Genomics Core (Room 2016) with printers are available to access MSDS information.
Hazardous Waste Disposal
  1. Biohazard waste including genetically modified organisms need to be contained and disposed of properly. You know what you work with and how it should be handled, so please take it back to your own lab and dispose of it there.
  2. The same holds true for chemical wastes that you may generate.  For wastes generated by the screening of chemical libraries, it is the user's responsibility for disposal according to Environmental Health and Safety guidelines.
  3. Biological materials used in chemical library screening in which pin tools (0.2 ml) are used for chemical transfer may be treated as biohazard waste and autoclaved.
  4. If you are uncertain about how to dispose of wastes you generate while working at IIGB facilities, contact the appropriate Academic Coordinator or the Academic Administrator.

DATA STORAGE AND MANAGEMENT

  1. Although for some instruments, IIGB Core facilities may maintain backups of data for operational purposes, the core facilities are not responsible for your data. Users must manage their instrument time, so that they are able to save data to flash drives or burn CDs and delete older data before their session ends.
  2. All equipment will have clearly labeled folders for user data. Users must save their data in a clearly named folder, which should not exceed 100 megabytes in size. If there is a memory shortage, the largest user files will be deleted first. Although we will try to inform users of any data deletion, it is ultimately the responsibility of users to save and manage their own data.
  3. Be aware that some systems run on Windows NT or 98, which lack support of USB memory devices. There are loaner zip discs available for transferring data to XP computers.
  4. In the Microscopy Core, CDs and paper sleeves are provided for burning data generated in that facility. In the Genomics Core, USB ports are available for all commonly-used instruments.

CITING

IIGB provides support leading to publications and grants. In order to gauge our effectiveness, and ultimately to expand our services to you, it is important that you acknowledge the use of the facilities and expertise in your publications and grants.

  1. All published images acquired at this facility should be cited as follows in the Acknowledgements:

    Images were generated at the Microscopy Core/Center for Plant Cell Biology at the Institute for Integrative Genome Biology at the University of California, Riverside.

  2. All published sequence, gene expression or other results facilitated by the Genomics Core should be cited as follows or similarly in Acknowledgements:

    DNA sequencing/quantitative PCR/Microarray data were generated by the Genomics Core at the Institute for Integrative Genome Biology at the University of California, Riverside.

  3. All published protein sequences, protein modifications, and related mass spectra data should be cited as follows in Acknowledgements:

    Proteomics and mass spectrometry experiments were conducted at the W. M. Keck Proteomics Laboratory/Center for Plant Cell Biology at the Institute for Integrative Genome Biology at the University of California, Riverside.

  4. All published analysis results and computational tool development projects facilitated by the staff and/or the computational resources in the bioinformatics core should be cited as follows or similarly in Acknowledgments:

    The data analysis (or modeling, software design, web,  database development, etc.) was performed in the Bioinformatics Core/Center for Plant Cell Biology at the Institute for Integrative Genome Biology at the University of California, Riverside.

  5. If research conducted in any of the Core Instrumentation Facilities results in published works, please provide a pdf file so that we can start building a knowledge base of local expertise.  If you get a cover page, we would be happy to frame it and put it on display. This information is essential in order to obtain resources for future support of your research.
  6. If you obtain a grant in which IIGB facilities or expertise is cited, it is crucial that you let us know and provide a cover page, so that we can track our contributions to your success. This information is essential in order to obtain resources for future support of your research.

CORE-SPECIFIC GUIDELINES

Microscopy Core

General

  1. Slides containing Arabidopsis seedlings may be disposed of in the sharps containers provided, which are destined for incineration. If you wish to bring in anything that other users may consider a potential hazard, contact the Academic Coordinator beforehand.
  2. Leave the work space clean and tidy. If any glass, (including cover slips) is dropped on the floor or inside an instrument, contact the Academic Coordinator for help removing it. Janitors will not clean glass-encrusted floors.
  3. In the unlikely event of an arc lamp bulb bursting in the Microscopy Core, leave the room immediately and wait 30 minutes for any mercury vapor to condense.

Cleanup and Shutdown

  1. Always clean all immersion lenses used in your session with a single folded kimwipe and blue cleaning fluid. Oil objectives need to be wiped 4 or 5 times to remove the more viscous oil. For dipping objectives, rinse with distilled water to remove sample buffer, then immerse in a large drop of blue cleaning fluid for 30 seconds, then wipe dry with a clean folded kimwipe. Dry objectives should not be cleaned. If an optic is contaminated, please contact David Carter for corrective action.
  2. When passing an instrument on to the next user, please indicate which lasers have been turned on and what other settings have been changed (e.g. pulsed laser mirror, DIC optics, black-out shutters). Shut down unwanted lasers yourself before leaving the instrument.
  3. Dust covers are an essential component of the microscope and must be in place when the instrument is not in use. Do not drop them on the dusty floor. Wait 5 minutes for an arc lamp to cool before covering with a dust cover, to avoid melting a hole.
Genomics Core

General

  1. All equipment for general usage by core customers can be reserved online at the IIGB Instrumentation Facilities Services and Billing site. If you wish to use a specific piece of equipment that is for general usage and not at the reservation site such as incubators, pipettes or robotic equipment, please consult with the Genomics Core staff. Please make such requests are made at least 24 hrs ahead of time since many items are in daily use by the Core.
  2. When requesting DNA sequencing services, please follow guidelines for submission of samples that are posted on our website. Online submissions forms are available at the IIGB Instrumentation Facilities Services and Billing site. Proper tubes or plates as well as proper labeling are important so that your samples are not lost or mislabeled.
  3. If you have not used a specific piece of equipment, please ask our staff for assistance. We provide training on an individual basis.
  4. For after-hour use, it is critical that any devices that use either lasers (Typhoon imager) or other light sources (qPCR systems) be shut off after use. This extends the lifetime of their light sources.
  5. We get many requests to purchase new equipment and/or offer new services. If you would like us to purchase a new piece of equipment or offer a new service, we are very interested in discussing this with you and are open to all possibilities. But please do your part by considering the following points.
    • Is this a piece of equipment or a service that others across campus will use or would it benefit only one lab or a very limited group?
    • If you feel that this piece of equipment or service is of broad interest, please speak to your colleagues and find out if there really is broad interest. Please remember that our goal is to use our available human and dollar resources to serve as many users as possible across campus.
  6. For trouble-shooting of DNA sequencing problems or other issues, please email the Genomics Core staff for assistance. Please remember that we are here to support your research efforts to the best of our abilities, but we do not know the details of your research nor can we guarantee your results.
Bioinformatics Core

General

  1. All computer hard- and software equipment in the bioinformatic facility can only be used with the official approval of the facility staff.  
  2. Accounts on our servers and high-performance computing systems need to be requested by email rakesh.kaundal@ucr.edu. A detailed listing of all resources including recharging rates is available at: http://bioinfo.ucr.edu.
  3. Access to the workstations in the bioinformatic core requires approval by  the director of the director of the facility.
  4. Training and consulting service need to be scheduled by email. Please send a service request with a short project description to rakesh.kaundal@ucr.edu.

Training

  1. The Bioinformatics Core provides training and consulting services on a wide spectrum of bioinformatic and cheminformatic topics. Detailed information about all workshop and training events is available on the Bioinformatics Workshop Page (http://facility.bioinformatics.ucr.edu/home/workshops). 
  2. The corresponding teaching material and manuals is available on our online Manual Page (http://manuals.bioinformatics.ucr.edu/).

Reserving Equipment

  • Almost all software and hardware in the Bioinformatics Core consists of networked multiuser resources, which can be accessed and utilized from anywhere at any time. Only very large projects that require more than 40GB of storage space or over 50 CPU cores need to be scheduled with the facility staff.
Proteomics Core

General

  1. All the users and visitors need be aware that the two major mass spectrometers in the Proteomics Core are operated with high voltage up to several thousands volts, which can be harmful and even lethal to humans. While in the lab, any users and visitors are restricted to the activity that they have been trained for and requested beforehand, and are not allowed to wander around in the lab out of curiosity.
  2. Please schedule an appointment with the Academic Coordinator to discuss scheduling experiments related to a research project and sample submission.
  1. To avoid potential cross-contamination, all users should prepare their samples in their own labs and are not allowed to use any enzymes, reagents, chemicals, and solvents from the core lab.
  2. Food and drink are not allowed in the Proteomics Core lab, and your assistance would be appreciated in maintaining a dust-free environment. Any instrument malfunction or problem should be reported to the Academic Coordinator. Users should not try to repair any instrumentation malfunctions.

More Information

General Campus Information

University of California, Riverside
900 University Ave.
Riverside, CA 92521
Tel: (951) 827-1012

Career OpportunitiesUCR Libraries
Campus StatusDirections to UCR

Genomics Information

Institute of Integrative Genome Biology
2150 Batchelor Hall

Tel: (951) 827-7177
E-mail: Aurelia Espinoza, Managing Director

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