UCR

Institute for Integrative Genome Biology



Members


Yinsheng WangYinsheng Wang

Professor of Chemistry 

Mailing Address:

Chemistry
Physical Sciences Unit 1 /332
University of California
Riverside, CA 92521

Phone: (951) 827-2700
Fax: (951) 827-4713
Email: yinsheng.wang@ucr.edu

Website
Lab Website
UCR Living the Promise (2013)

Degree(s):

D 2001 Washington University, St. Louis
MS 1996 Dalian Institute of Chemical Physics, China
BS 1993 Shandong University, China

College/Division Affiliation:

College of Natural and Agricultural Sciences

Center/Inst Affiliation(s):

Center for Plant Cell Biology

Areas Of Expertise:

Oxidative DNA Damage; Covalent Modifications of Proteins

Awards / Honors:

2013 Biemann Medal, American Society for Mass Spectrometry (ASMS)
2005 ASMS (American Society for Mass Spectrometry) Research Award

Research Summary:

Our research focuses on using mass spectrometry (MS) to understand important biological problems, and we are particularly interested in DNA damage induced by reactive oxygen species and covalent modifications of proteins.

Oxidative DNA Damage
In this research area, we employ a plethora of approaches, which include analytical chemistry, synthetic organic chemistry, biochemistry, and molecular biology, to understand the biological implications of oxidative DNA damage at the molecular level. Our emphasis has been placed on the identification, structure elucidation, and quantification of a class of oxidative DNA lesions where two neighboring nucleobases in the same strand are covalently linked. In this respect, we have been able to characterize the structures of a number of novel oxidative intrastrand crosslink lesions by mass spectrometric and NMR spectroscopic methods. In addition, we quantify the amounts of DNA lesions formed from a variety of oxidation conditions by LC-MS/MS with isotope-incorporated standards. Moreover, we characterize the biophysical and biochemical properties of oxidative DNA Lesions, and we are particularly interested in investigating the thermodynamic as well as the in vitro and in vivo mutagenic properties of various oxidative DNA lesions.

Covalent Modifications of Proteins
Current projects in this area encompass the post-translational modifications of chromosomal high-mobility group (HMG) proteins, histones, DNA repair enzymes, and methyl-CpG binding proteins. In this respect, we use mass spectrometry for the identification of the sites and types of modifications, and we also examine the biological functions of these modifications. We have in our laboratory a ThermoFinnigan Deca XP ion trap and an IonSpec FT-ICR mass spectrometer. We also have access to an Applied Biosystems Voyager DE-STR MALDI-TOF and a Micromass Global Ultima Q-TOF mass spectrometers in the Analytical Chemistry Instrumentation Facility as well as a Q-STAR XL O-MALDI Q-TOF mass spectrometer in the W. M. Keck Proteomics Laboratory. Students in our lab have the opportunity to gain hands-on experience with various state-of-the-art mass spectrometers. Depending on the nature of individual research project, they are also exposed to synthetic organic chemistry, biochemistry, molecular biology, and molecular modeling.

Related Press Releases:

Selected Publications:

List of publications from HubMed and Personal Website 

Lab Personnel:

Dong, Xiaoli
Postgraduate Researcher —   
Wang, Jin
Postgraduate Researcher — 
You, Changjun
Postgraduate Researcher —
Yuan, Bifeng
Postgraduate Researcher —
Andersen, Nisana
Graduate Student Researcher —
Fu, Lijuan
Graduate Student Researcher — 
Guerrero , Candace
Graduate Student Researcher —
Jiang, Yong
Graduate Student Researcher — 
Swanson, Ashley
Graduate Student Researcher —
Vargas, Mario
Graduate Student Researcher —
Wang, Hongxia
Graduate Student Researcher —
Wang, Jianshuang
Graduate Student Researcher — 
Williams, Renee
Graduate Student Researcher —
Xiao, Yongsheng
Graduate Student Researcher — 
Xiong, Lei
Graduate Student Researcher —
Zhang, Fan
Graduate Student Researcher — 
Zhang, Jing
Graduate Student Researcher —
Zheng, Xueyun
Graduate Student Researcher —
Williams, Preston
Graduate Student Researcher —

More Information

General Campus Information

University of California, Riverside
900 University Ave.
Riverside, CA 92521
Tel: (951) 827-1012

Career OpportunitiesUCR Libraries
Campus StatusDirections to UCR

Genomics Information

Institute of Integrative Genome Biology
2150 Batchelor Hall

Tel: (951) 827-7177
E-mail: Aurelia Espinoza, Managing Director

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